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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COA4 Recombinant antibody (83326-1-RR)

    WB analysis using 83326-1-RR (same clone as 83326-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83326-1-RR (COA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83326-1-RR (same clone as 83326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83326-1-RR (COA4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human ovarian cancer using 83326-1-RR (same clone as 83326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human ovarian cancer using 83326-1-RR (same clone as 83326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83326-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83326-1-RR

    Affinity and Kinetic Characterization of 83326-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83326-1-RR against Human COA4 were performed. The affinity constant is 0.307 nM.

COA4 Recombinant monoclonal antibody, PBS Only

COA4 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83326-1-PBS
Clone No.230070G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CHCHD8, E2IG2, E2 induced gene 2 protein, 230070G9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83326-1-PBS targets COA4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33966

Product name: Recombinant human CHCHD8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of NM_016565.2

Sequence: MSTSVPQGHTWTQRVKKDDEEEDPLDQLISRSGCAASHFAVQECMAQHQDWRQCQPQVQAFKDCMSEQQARRQEELQRRQEQAGAHH

相同性解析による交差性が予測される生物種
Full Name Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
Calculated molecular weight10 kDa
Observed molecular weight10 kDa
GenBank accession numberNM_016565.2
Gene Symbol COA4
Gene ID (NCBI) 51287
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYJ1-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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