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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNIH4 Polyclonal antibody (15015-1-AP)

    WB analysis using 15015-1-AP (same clone as 15015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15015-1-AP (CNIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15015-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CNIH4 Polyclonal antibody (15015-1-AP)

    IF Staining of mouse testis using 15015-1-AP (same clone as 15015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15015-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CNIH4 Polyclonal antibody (15015-1-AP)

    IF Staining of mouse testis using 15015-1-AP (same clone as 15015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15015-1-PBS in a different storage buffer formulation.

CNIH4 Polyclonal antibody, PBS Only

CNIH4 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 15015-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

CNIH-4, Cornichon family AMPA receptor auxiliary protein 4, HSPC163, Protein cornichon homolog 4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15015-1-PBS targets CNIH4 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6965

Product name: Recombinant human CNIH4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC000573

Sequence: MEAVVFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTVLLLMSLHWFIFLLNLPVATWNIYRYIMVPSGNMGVFDPTEIHNRGQLKSHMKEAMIKLG

相同性解析による交差性が予測される生物種
Full Name cornichon homolog 4 (Drosophila)
Calculated molecular weight 16 kDa
Observed molecular weight16-18 kDa
GenBank accession numberBC000573
Gene Symbol CNIH4
Gene ID (NCBI) 29097
RRIDAB_3741857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P003
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CNIH4 (Cornichon Family Member 4) is a protein-coding gene belonging to the cornichon family, which plays a critical role in regulating G protein-coupled receptor (GPCR) trafficking from the endoplasmic reticulum to the cell surface. It facilitates GPCR export through the early secretory pathway and is regulated by TMED9.

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