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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNIH4 Polyclonal antibody (15015-1-AP)

    WB analysis using 15015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15015-1-AP (CNIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CNIH4 Polyclonal antibody (15015-1-AP)

    IF Staining of mouse testis using 15015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CNIH4 Polyclonal antibody (15015-1-AP)

    IF Staining of mouse testis using 15015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CNIH4 Polyclonal antibody

CNIH4 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 15015-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CNIH-4, Cornichon family AMPA receptor auxiliary protein 4, HSPC163, Protein cornichon homolog 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15015-1-AP targets CNIH4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNIH4 fusion protein Ag6965 相同性解析による交差性が予測される生物種
Full Name cornichon homolog 4 (Drosophila)
Calculated molecular weight 16 kDa
Observed molecular weight16-18 kDa
GenBank accession numberBC000573
Gene Symbol CNIH4
Gene ID (NCBI) 29097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P003
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNIH4 (Cornichon Family Member 4) is a protein-coding gene belonging to the cornichon family, which plays a critical role in regulating G protein-coupled receptor (GPCR) trafficking from the endoplasmic reticulum to the cell surface. It facilitates GPCR export through the early secretory pathway and is regulated by TMED9.

Protocols

Product Specific Protocols
WB protocol for CNIH4 antibody 15015-1-APDownload protocol
IF protocol for CNIH4 antibody 15015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

World J Gastroenterol

Genome-wide map of N6-methyladenosine circular RNAs identified in mice model of severe acute pancreatitis.

Authors - Jun Wu
View Article
humanIHC

Biomedicines

CLIC4 Is a New Biomarker for Glioma Prognosis

Authors - Zhichun Liu
View Article