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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CMAS Polyclonal antibody (13006-1-AP)

    IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CMAS Polyclonal antibody (13006-1-AP)

    IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CMAS Polyclonal antibody (13006-1-AP)

    IF Staining of A431 using 13006-1-AP (same clone as 13006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody (13006-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

CMAS Polyclonal antibody, PBS Only

CMAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13006-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, EC:2.7.7.43

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13006-1-PBS targets CMAS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3686

Product name: Recombinant human CMAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC016609

Sequence: MDSVEKGAATSVSNPRGRPSRGRPPKLQRNSRGGQGRGVEKPPHLAALILARGGSKGIPLKNIKHLAGVPLIGWVLRAALDSGAFQSVWVSTDHDEIENVAKQFGAQVHRRSSEVSKDSSTSLDAIIEFLNYHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLR

相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate N-acetylneuraminic acid synthetase
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC016609
Gene Symbol CMAS
Gene ID (NCBI) 55907
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFW8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

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