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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CMAS Polyclonal antibody (13006-1-AP)

    IHC staining of human placenta using 13006-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CMAS Polyclonal antibody (13006-1-AP)

    IHC staining of human placenta using 13006-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CMAS Polyclonal antibody (13006-1-AP)

    IF Staining of A431 using 13006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody (13006-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CMAS Polyclonal antibody

CMAS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13006-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, EC:2.7.7.43

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat pancreas tissue, mouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13006-1-AP targets CMAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3686

Product name: Recombinant human CMAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC016609

Sequence: MDSVEKGAATSVSNPRGRPSRGRPPKLQRNSRGGQGRGVEKPPHLAALILARGGSKGIPLKNIKHLAGVPLIGWVLRAALDSGAFQSVWVSTDHDEIENVAKQFGAQVHRRSSEVSKDSSTSLDAIIEFLNYHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLR

相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate N-acetylneuraminic acid synthetase
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC016609
Gene Symbol CMAS
Gene ID (NCBI) 55907
RRIDAB_3085404
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

Protocols

Product Specific Protocols
IF protocol for CMAS antibody 13006-1-APDownload protocol
IHC protocol for CMAS antibody 13006-1-APDownload protocol
WB protocol for CMAS antibody 13006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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