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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CLOCK Polyclonal antibody (18094-1-AP)

    WB analysis of HEK-293 using 18094-1-AP (same clone as 18094-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLOCK Polyclonal antibody (18094-1-AP)

    WB analysis using 18094-1-AP (same clone as 18094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLOCK Polyclonal antibody (18094-1-AP)

    WB analysis of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLOCK Polyclonal antibody (18094-1-AP)

    WB analysis of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLOCK Polyclonal antibody (18094-1-AP)

    IP experiment of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    IP result of anti-CLOCK (IP:18094-1-AP, 4ug; Detection:18094-1-AP 1:300) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CLOCK Polyclonal antibody (18094-1-AP)

    IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CLOCK Polyclonal antibody (18094-1-AP)

    IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLOCK Polyclonal antibody (18094-1-AP)

    IF Staining of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    Immunofluorescent analysis of HeLa cells using 18094-1-AP (CLOCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CLOCK Polyclonal antibody, PBS Only

CLOCK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18094-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18094-1-PBS targets CLOCK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12826

Product name: Recombinant human CLOCK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC041878

Sequence: MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDKSTVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFISNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTYEYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIWL

相同性解析による交差性が予測される生物種
Full Name clock homolog (mouse)
Calculated molecular weight 846 aa, 95 kDa
Observed molecular weight 95-110 kDa
GenBank accession numberBC041878
Gene Symbol CLOCK
Gene ID (NCBI) 9575
RRIDAB_2878497
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15516
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

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