Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 18094-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 18094-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 18094-1-AP
Mouse kidney tissues were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18094-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18094-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18094-1-AP
IP result of anti-CLOCK (IP:18094-1-AP, 4ug; Detection:18094-1-AP 1:300) with HeLa cells lysate 1600 ug.
IHC staining of human liver using 18094-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18094-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18094-1-AP
Immunofluorescent analysis of HeLa cells using 18094-1-AP (CLOCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CLOCK Polyclonal antibody

CLOCK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18094-1-AP

Publications(31)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48, KAT13D

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (31)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse kidney tissue, PC-3 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 29 publications below
IHC	See 4 publications below
IF	See 5 publications below
ChIP	See 1 publications below

Product Information

18094-1-AP targets CLOCK in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12826 Product name: Recombinant human CLOCK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC041878 Sequence: MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDKSTVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFISNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTYEYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIWL 相同性解析による交差性が予測される生物種
Full Name	clock homolog (mouse)
Calculated molecular weight	846 aa, 95 kDa
Observed molecular weight	95-110 kDa
GenBank accession number	BC041878
Gene Symbol	CLOCK
Gene ID (NCBI)	9575
RRID	AB_2878497
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15516
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

Protocols

Product Specific Protocols
IF protocol for CLOCK antibody 18094-1-AP	Download protocol
IHC protocol for CLOCK antibody 18094-1-AP	Download protocol
IP protocol for CLOCK antibody 18094-1-AP	Download protocol
WB protocol for CLOCK antibody 18094-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A druggable copper-signalling pathway that drives inflammation Authors - Stéphanie Solier View Article
human	WB	Eur Heart J Circadian nuclear receptor Rev-erbα is expressed by platelets and potentiates platelet activation and thrombus formation Authors - Jianfeng Shi View Article
mouse,human	WB	Cell Host Microbe A CLOCK-targeting lncRNA induces trained immunity against tuberculosis. Authors - Shanshan Yu View Article
human	WB	Nat Commun The rhythmic coupling of Egr-1 and Cidea regulates age-related metabolic dysfunction in the liver of male mice Authors - Jing Wu View Article
human	WB	Cell Death Dis hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway. Authors - Xinxing Wan View Article
mouse	WB	J Neuroinflammation Repetitive unidirectional spinal tactile stimulation engages microglial Bmal1 pathways to promote synaptic remodeling in the mPFC of adolescent VPA-exposed mice. Authors - Yi-Nan Chen View Article

Validation Data Gallery

WB analysis of HEK-293 using 18094-1-AP

WB analysis using 18094-1-AP

WB analysis using 18094-1-AP

WB analysis of HeLa using 18094-1-AP

WB analysis of HeLa using 18094-1-AP

IP experiment of HeLa using 18094-1-AP

IHC staining of human liver using 18094-1-AP

IHC staining of human liver using 18094-1-AP

IF Staining of HeLa using 18094-1-AP

CLOCK Polyclonal antibody

CLOCK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A druggable copper-signalling pathway that drives inflammation

Circadian nuclear receptor Rev-erbα is expressed by platelets and potentiates platelet activation and thrombus formation

A CLOCK-targeting lncRNA induces trained immunity against tuberculosis.

The rhythmic coupling of Egr-1 and Cidea regulates age-related metabolic dysfunction in the liver of male mice

hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.

Repetitive unidirectional spinal tactile stimulation engages microglial Bmal1 pathways to promote synaptic remodeling in the mPFC of adolescent VPA-exposed mice.

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