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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis using 23839-1-AP (same clone as 23839-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of rat kidney using 23839-1-AP (same clone as 23839-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of MCF-7 using 23839-1-AP (same clone as 23839-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of U-937 using 23839-1-AP (same clone as 23839-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of mouse kidney using 23839-1-AP (same clone as 23839-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CLIP1 Polyclonal antibody (23839-1-AP)

    WB analysis of MCF-7 using 23839-1-AP (same clone as 23839-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLIP1 Polyclonal antibody (23839-1-AP)

    IP experiment of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

    IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLIP1 Polyclonal antibody (23839-1-AP)

    IF Staining of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIP1 antibody (23839-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

CLIP1 Polyclonal antibody, PBS Only

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 23839-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23839-1-PBS targets CLIP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20811

Product name: Recombinant human CLIP1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1090-1438 aa of BC126305

Sequence: DAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF

相同性解析による交差性が予測される生物種
Full Name CAP-GLY domain containing linker protein 1
Calculated molecular weight 1438 aa, 162 kDa
Observed molecular weight 160-170 kDa, 135 kDa
GenBank accession numberBC126305
Gene Symbol CLIP1
Gene ID (NCBI) 6249
RRIDAB_2879334
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDP30622
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CLIP1, also known as RSN, CYLN1, is a member of the microtubule (MT) plus-end tracking protein family. CLIP1 associates with the ends of growing MTs. These proteins regulate MT dynamic behavior and are important for MT-mediated transport over the length of axons and dendrites. It has an important role in spermatogenesis as well as in neuronal development(PMID: 24569606).

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