Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 23839-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23839-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23839-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 23839-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 23839-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23839-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23839-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 23839-1-AP
IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug.
IF Staining of HeLa using 23839-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIP1 antibody (23839-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

CLIP1 Polyclonal antibody

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 23839-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IF	See 2 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

23839-1-AP targets CLIP1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20811 Product name: Recombinant human CLIP1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1090-1438 aa of BC126305 Sequence: DAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF 相同性解析による交差性が予測される生物種
Full Name	CAP-GLY domain containing linker protein 1
Calculated molecular weight	1438 aa, 162 kDa
Observed molecular weight	160-170 kDa, 135 kDa
GenBank accession number	BC126305
Gene Symbol	CLIP1
Gene ID (NCBI)	6249
RRID	AB_2879334
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P30622
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CLIP1, also known as RSN, CYLN1, is a member of the microtubule (MT) plus-end tracking protein family. CLIP1 associates with the ends of growing MTs. These proteins regulate MT dynamic behavior and are important for MT-mediated transport over the length of axons and dendrites. It has an important role in spermatogenesis as well as in neuronal development(PMID: 24569606).

Protocols

Product Specific Protocols
IF protocol for CLIP1 antibody 23839-1-AP	Download protocol
IP protocol for CLIP1 antibody 23839-1-AP	Download protocol
WB protocol for CLIP1 antibody 23839-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human, mouse	IF	Sci Bull (Beijing) The mechanisms of MTOCs maturation in human and mouse oocytes. Authors - Hao Gu View Article
rat,human	WB,ELISA	Exp Mol Med Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver Authors - Pengpeng Yue View Article
		Cell Death Differ USP21-mediated deubiquitylation stimulates NuMA recruitment to the cell cortex to promote mitotic spindle orientation. Authors - Mingzheng Hu View Article
human	WB	Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration Authors - Yunfeng Hu View Article
mouse	WB	Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. Authors - Chuan Wang View Article

Validation Data Gallery

WB analysis using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of rat kidney using 23839-1-AP

WB analysis of MCF-7 using 23839-1-AP

WB analysis of U-937 using 23839-1-AP

WB analysis of mouse kidney using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of HeLa using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of MCF-7 using 23839-1-AP

IP experiment of HeLa using 23839-1-AP

IF Staining of HeLa using 23839-1-AP

CLIP1 Polyclonal antibody

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The mechanism of acentrosomal spindle assembly in human oocytes

The mechanisms of MTOCs maturation in human and mouse oocytes.

Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver

USP21-mediated deubiquitylation stimulates NuMA recruitment to the cell cortex to promote mitotic spindle orientation.

Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

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