Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rat spleen tissue, HL-60 cells, C2C12 cells, RAW 264.7 cells, C6 cells, mouse spleen tissue |
| Positive IHC detected in | mouse kidney tissue, rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85831-4-RR targets CLIC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CLIC1 fusion protein Ag6049 相同性解析による交差性が予測される生物種 |
| Full Name | chloride intracellular channel 1 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 27-32 kDa |
| GenBank accession number | BC064527 |
| Gene Symbol | CLIC1 |
| Gene ID (NCBI) | 1192 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00299 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLIC1 antibody 85831-4-RR | Download protocol |
| IHC protocol for CLIC1 antibody 85831-4-RR | Download protocol |
| IF protocol for CLIC1 antibody 85831-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |