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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLIC1 Recombinant monoclonal antibody (85831-4-RR)

    WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLIC1 Recombinant monoclonal antibody (85831-4-RR)

    WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLIC1 Recombinant monoclonal antibody (85831-4-RR)

    IHC staining of mouse kidney using 85831-4-RR (same clone as 85831-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CLIC1 Recombinant monoclonal antibody (85831-4-RR)

    IHC staining of rat spleen using 85831-4-RR (same clone as 85831-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLIC1 Recombinant monoclonal antibody (85831-4-RR)

    IF Staining of HeLa using 85831-4-RR (same clone as 85831-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85831-4-RR

    Affinity and Kinetic Characterization of 85831-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85831-4-RR against Human CLIC1 were performed. The affinity constant is 22.0 nM.

CLIC1 Recombinant monoclonal antibody, PBS Only

CLIC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85831-4-PBS
Clone No.250143E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

G6, NCC27

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85831-4-PBS targets CLIC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6049

Product name: Recombinant human CLIC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC064527

Sequence: MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK

相同性解析による交差性が予測される生物種
Full Name chloride intracellular channel 1
Calculated molecular weight 27 kDa
Observed molecular weight27-32 kDa
GenBank accession numberBC064527
Gene Symbol CLIC1
Gene ID (NCBI) 1192
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00299
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).

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