Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-16424 targets CHMP7 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9625 Product name: Recombinant human CHMP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-453 aa of BC019110 Sequence: ELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAELEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL 相同性解析による交差性が予測される生物種 |
| Full Name | CHMP family, member 7 |
| Calculated molecular weight | 453 aa, 51 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC019110 |
| Gene Symbol | CHMP7 |
| Gene ID (NCBI) | 91782 |
| RRID | AB_3672651 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUX9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP7 (Charged multivesicular body protein 7), it is expected to be located in the nucleus and cytoplasm, which is ubiquitinated in lymph node and appendix. It is reported that Lem2p (LEM2), a member of the LEM (Lap2- Emerin-Man1) family of inner nuclear membrane proteins, and the ESCRT-II/ESCRT- III hybrid protein Cmp7p (CHMP7), work together to recruit additional ESCRT-III proteins to holes in the nuclear membrane. CHMP7 protein is involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. It is a part of ESCRT III complex, which colocalizes with chromatin. The molecular weight of CHMP7 is 50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CHMP7 antibody CL488-16424 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
