Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-86474-3 targets CHMP6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9357 Product name: Recombinant human CHMP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC010108 Sequence: MGNLFGRKKQSRVTEQDKAILQLKQQRDKLRQYQKRIAQQLERERALARQLLRDGRKERAKLLLKKKRYQEQLLDRTENQISSLEAMVQSIEFTQIEMKVMEGLQFGNECLNKMHQVMSIEEVERILDETQEAVEYQRQIDELLAGSFTQEDEDAILEELSAITQEQIELPEVPSEPLPEKIPENVPVKARPRQAELVAAS 相同性解析による交差性が予測される生物種 |
| Full Name | chromatin modifying protein 6 |
| Calculated molecular weight | 201 aa, 23 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC010108 |
| Gene Symbol | CHMP6 |
| Gene ID (NCBI) | 79643 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96FZ7 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP6 (charged multivesicular body protein 6) is a protein that plays a significant role in the formation of multivesicular bodies (MVBs) within cells. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which is involved in the sorting and transport of proteins within the cell. CHMP6 has been identified as a novel prognostic biomarker in bladder cancer, where it enhances cell survival and migration by regulating the cell cycle. (PMID: 40630215)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CHMP6 antibody CL488-86474-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |