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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Monoclonal antibody (68811-2-Ig)

    WB analysis using 68811-2-Ig (same clone as 68811-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68811-2-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68811-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CHMP4B Monoclonal antibody (68811-2-Ig)

    IF Staining of HCT 116 using 68811-2-Ig (same clone as 68811-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-2-Ig, Clone: 2A3F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68811-2-PBS in a different storage buffer formulation.

CHMP4B Monoclonal antibody, PBS Only

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68811-2-PBS
Clone No.2A3F5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68811-2-PBS targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33487

Product name:

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
GenBank accession numberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H444
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.