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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Monoclonal antibody (68811-2-Ig)

    WB analysis using 68811-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68811-2-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CHMP4B Monoclonal antibody (68811-2-Ig)

    IF Staining of HCT 116 using 68811-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-2-Ig, Clone: 2A3F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

CHMP4B Monoclonal antibody

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68811-2-Ig
Clone No.2A3F5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68811-2-Ig targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33487

Product name:

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
RRIDAB_3670433
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Protocols

Product Specific Protocols
WB protocol for CHMP4B antibody 68811-2-IgDownload protocol
IF protocol for CHMP4B antibody 68811-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols