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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Properdin/PFC Monoclonal antibody (66788-1-Ig)

    WB analysis of Jurkat using 66788-1-Ig (same clone as 66788-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66788-1-Ig (CFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66788-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Properdin/PFC Monoclonal antibody (66788-1-Ig)

    WB analysis of human plasma using 66788-1-Ig (same clone as 66788-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66788-1-Ig (CFP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66788-1-PBS in a different storage buffer formulation.

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Properdin/PFC Monoclonal antibody, PBS Only

Properdin/PFC Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66788-1-PBS
Clone No.1D5A9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, Indirect ELISA

Synonyms

CFP, PFC, complement factor properdin, Complement factor P, BFD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66788-1-PBS targets Properdin/PFC in WB, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Properdin/PFC fusion protein Ag9798 相同性解析による交差性が予測される生物種
Full Name complement factor properdin
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 50 kDa, 100 kDa, 150 kDa
GenBank accession numberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
RRIDAB_2882133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27918
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.