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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CFP Monoclonal antibody (66788-1-Ig)

    WB analysis of Jurkat using 66788-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66788-1-Ig (CFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using CFP Monoclonal antibody (66788-1-Ig)

    WB analysis of human plasma using 66788-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66788-1-Ig (CFP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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CFP Monoclonal antibody

CFP Monoclonal Antibody for WB, ELISA
Cat No. 66788-1-Ig
Clone No.1D5A9

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, ELISA

Synonyms

BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, human plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66788-1-Ig targets CFP in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CFP fusion protein Ag9798 相同性解析による交差性が予測される生物種
Full Name complement factor properdin
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 50 kDa, 100 kDa, 150 kDa
GenBank accession numberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
RRIDAB_2882133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.

Protocols

Product Specific Protocols
WB protocol for CFP antibody 66788-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols