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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CEP97 Polyclonal antibody (22050-1-AP)

    WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CEP97 Polyclonal antibody (22050-1-AP)

    WB analysis of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CEP97 Polyclonal antibody (22050-1-AP)

    WB analysis of HEK-293 using 22050-1-AP (same clone as 22050-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CEP97 Polyclonal antibody (22050-1-AP)

    WB analysis of PC-3 using 22050-1-AP (same clone as 22050-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CEP97 Polyclonal antibody (22050-1-AP)

    IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

    IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CEP97 Polyclonal antibody (22050-1-AP)

    IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CEP97 Polyclonal antibody (22050-1-AP)

    IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CEP97 Polyclonal antibody (22050-1-AP)

    IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CEP97 Polyclonal antibody (22050-1-AP)

    IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CEP97 Polyclonal antibody (22050-1-AP)

    IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP97 antibody (22050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

CEP97 Polyclonal antibody, PBS Only

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22050-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22050-1-PBS targets CEP97 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16995

Product name: Recombinant human CEP97 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 506-865 aa of BC041085

Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV

相同性解析による交差性が予測される生物種
Full Name centrosomal protein 97kDa
Calculated molecular weight 865 aa, 97 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC041085
Gene Symbol CEP97
Gene ID (NCBI) 79598
RRIDAB_11182378
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IW35
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

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