Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HL-60 using 22050-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22050-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22050-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 22050-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22050-1-AP
IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human breast cancer using 22050-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 22050-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22050-1-AP (CEP97 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22050-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22050-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22050-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MDCK using 22050-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP97 antibody (22050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CEP97 Polyclonal antibody

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22050-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HL-60 cells, PC-3 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human liver tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IF	See 18 publications below
IP	See 2 publications below

Product Information

22050-1-AP targets CEP97 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity	human, canine
Cited Reactivity	human, mouse, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16995 Product name: Recombinant human CEP97 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 506-865 aa of BC041085 Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV 相同性解析による交差性が予測される生物種
Full Name	centrosomal protein 97kDa
Calculated molecular weight	865 aa, 97 kDa
Observed molecular weight	130-140 kDa
GenBank accession number	BC041085
Gene Symbol	CEP97
Gene ID (NCBI)	79598
RRID	AB_11182378
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IW35
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

Protocols

Product Specific Protocols
IF protocol for CEP97 antibody 22050-1-AP	Download protocol
IHC protocol for CEP97 antibody 22050-1-AP	Download protocol
IP protocol for CEP97 antibody 22050-1-AP	Download protocol
WB protocol for CEP97 antibody 22050-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Time-series reconstruction of the molecular architecture of human centriole assembly Authors - Marine H Laporte View Article
mouse	WB	Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. Authors - Min Liu View Article
human	WB	Protein Cell Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1. Authors - Hongmei Mao View Article
mouse,human	WB,IF	Nat Commun M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. Authors - Ning Huang View Article
human	WB	J Clin Invest Primary cilia formation requires the Leigh syndrome-associated mitochondrial protein NDUFAF2 Authors - Chien-Hui Lo View Article
		Cell Death Dis TUFT1 regulates cancer progression by suppressing centrosome amplification and mitotic spindle multipolarity Authors - Shaojie Feng View Article

Validation Data Gallery

WB analysis of HL-60 using 22050-1-AP

WB analysis using 22050-1-AP

WB analysis of HeLa using 22050-1-AP

WB analysis of HEK-293 using 22050-1-AP

WB analysis of PC-3 using 22050-1-AP

IP experiment of HeLa using 22050-1-AP

IHC staining of human breast cancer using 22050-1-AP

IHC staining of human stomach cancer using 22050-1-AP

IHC staining of human breast cancer using 22050-1-AP

IHC staining of human liver using 22050-1-AP

IHC staining of human liver using 22050-1-AP

IF Staining of MDCK using 22050-1-AP

CEP97 Polyclonal antibody

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Time-series reconstruction of the molecular architecture of human centriole assembly

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Primary cilia formation requires the Leigh syndrome-associated mitochondrial protein NDUFAF2

TUFT1 regulates cancer progression by suppressing centrosome amplification and mitotic spindle multipolarity

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