Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-24428 targets CEP135 in IF/ICC applications and shows reactivity with human, Canine samples.
| Tested Reactivity | human, Canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19122 Product name: Recombinant human CEP135 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-233 aa of BC136535 Sequence: MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQ 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 135kDa |
| Calculated molecular weight | 1140 aa, 134 kDa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | BC136535 |
| Gene Symbol | CEP135 |
| Gene ID (NCBI) | 9662 |
| RRID | AB_3084080 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q66GS9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CEP135 antibody CL488-24428 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
