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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR

    human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CEACAM8/CD66b Recombinant monoclonal antibody (83105-6-RR)

    IHC staining of human tonsillitis using 83105-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83105-6-RR (CEACAM8/CD66b antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CEACAM8/CD66b Recombinant monoclonal antibody (83105-6-RR)

    IHC staining of human tonsillitis using 83105-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83105-6-RR (CEACAM8/CD66b antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Transfected CHO-K1 using CEACAM8/CD66b Recombinant antibody (83105-6-RR)

    IF Staining of Transfected CHO-K1 using 83105-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using CEACAM8 antibody (83105-6-RR, Clone: 242954B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83105-6-RR

    Affinity and Kinetic Characterization of 83105-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 14.1 nM.

CEACAM8/CD66b Recombinant monoclonal antibody

CEACAM8/CD66b Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83105-6-RR
Clone No.242954B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman saliva, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTransfected CHO-K1

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83105-6-RR targets CEACAM8/CD66b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg32133

Product name: Recombinant Human CEACAM-8 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 35-319 aa of BC026263

Sequence: QLTIEAVPSNAAEGKEVLLLVHNLPQDPRGYNWYKGETVDANRRIIGYVISNQQITPGPAYSNRETIYPNASLLMRNVTRNDTGSYTLQVIKLNLMSEEVTGQFSVHPETPKPSISSNNSNPVEDKDAVAFTCEPETQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLLSVTRNDVGPYECEIQNPASANFSDPVTLNVLYGPDAPTISPSDTYYHAGVNLNLSCHAASNPPSQYSWSVNGTFQQYTQKLFIPNITTKNSGSYACHTTNSATGRNRTTVRMITVS

相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated molecular weight 38 kDa
Observed molecular weight95-100kDa
GenBank accession numberBC026263
Gene Symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP31997
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).

Protocols

Product Specific Protocols
IF protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
IHC protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
WB protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
Standard Protocols
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