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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR (same clone as 83105-6-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83105-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR (same clone as 83105-6-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83105-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83105-6-RR

    Affinity and Kinetic Characterization of 83105-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 13.9 nM.

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CEACAM8 Recombinant antibody, PBS Only

CEACAM8 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83105-6-PBS
Clone No.242954B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CD66b, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b, CD 67

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83105-6-PBS targets CEACAM8 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CEACAM8 fusion protein Eg32133 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated molecular weight 38 kDa
Observed molecular weight95-100kDa
GenBank accession numberBC026263
Gene Symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31997
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).