Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16615-1-AP (same clone as 16615-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 16615-1-AP (same clone as 16615-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16615-1-AP (same clone as 16615-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 16615-1-AP (same clone as 16615-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16615-1-AP (same clone as 16615-1-PBS)
IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16615-1-AP (same clone as 16615-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16615-1-AP (same clone as 16615-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDKN2AIP antibody (16615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16615-1-PBS in a different storage buffer formulation.

CDKN2AIP Polyclonal antibody, PBS Only

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16615-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CARF, CDKN2A-interacting protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16615-1-PBS targets CDKN2AIP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9907 Product name: Recombinant human CDKN2AIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-579 aa of BC022270 Sequence: ASEAEAPDKHGSSFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL 相同性解析による交差性が予測される生物種
Full Name	CDKN2A interacting protein
Calculated molecular weight	579 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC022270
Gene Symbol	CDKN2AIP
Gene ID (NCBI)	55602
RRID	AB_2244775
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NXV6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16615-1-AP (same clone as 16615-1-PBS)

WB analysis of Jurkat using 16615-1-AP (same clone as 16615-1-PBS)

WB analysis of HeLa using 16615-1-AP (same clone as 16615-1-PBS)

WB analysis of Jurkat using 16615-1-AP (same clone as 16615-1-PBS)

IP experiment of HeLa using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of human placenta using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of human placenta using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of human stomach cancer using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of human stomach cancer using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of mouse liver using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of mouse liver using 16615-1-AP (same clone as 16615-1-PBS)

IHC staining of mouse testis using 16615-1-AP (same clone as 16615-1-PBS)