Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16615-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16615-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16615-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16615-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16615-1-AP
IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human kidney using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16615-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 16615-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDKN2AIP antibody (16615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CDKN2AIP Polyclonal antibody

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16615-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CDKN2A-interacting protein, CARF

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, Jurkat cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

16615-1-AP targets CDKN2AIP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9907 Product name: Recombinant human CDKN2AIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-579 aa of BC022270 Sequence: ASEAEAPDKHGSSFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL 相同性解析による交差性が予測される生物種
Full Name	CDKN2A interacting protein
Calculated molecular weight	579 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC022270
Gene Symbol	CDKN2AIP
Gene ID (NCBI)	55602
RRID	AB_2244775
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NXV6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CDKN2AIP antibody 16615-1-AP	Download protocol
IHC protocol for CDKN2AIP antibody 16615-1-AP	Download protocol
IP protocol for CDKN2AIP antibody 16615-1-AP	Download protocol
WB protocol for CDKN2AIP antibody 16615-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC,IF	Cell Biosci CDKN2AIP is critical for spermiogenesis and germ cell development Authors - Yuming Cao View Article
mouse	IF	Acta Biochim Biophys Sin (Shanghai) CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1 Authors - Yuming Cao View Article
human	WB	Clin Cosmet Investig Dermatol Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network. Authors - Xianhong Yang View Article
mouse	WB,CoIP	J Diabetes Taurine Alleviates Pancreatic β-Cell Senescence by Inhibition of p53 Pathway Authors - Baomin Wang View Article
mouse	WB,IF	Int J Cardiol Reduced myocardial CARF and the underlying implications in left ventricular noncompaction cardiomyopathy Authors - Liu Hui KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16615-1-AP

WB analysis of Jurkat using 16615-1-AP

WB analysis of HeLa using 16615-1-AP

WB analysis of Jurkat using 16615-1-AP

IP experiment of HeLa using 16615-1-AP

IHC staining of human kidney using 16615-1-AP

IHC staining of human placenta using 16615-1-AP

IHC staining of human testis using 16615-1-AP

IHC staining of human testis using 16615-1-AP

IHC staining of human skin using 16615-1-AP

IHC staining of human skin using 16615-1-AP

IHC staining of human liver using 16615-1-AP

IHC staining of human liver using 16615-1-AP

IHC staining of human spleen using 16615-1-AP

IHC staining of human spleen using 16615-1-AP

IHC staining of human ovary using 16615-1-AP

IHC staining of human kidney using 16615-1-AP

IHC staining of human placenta using 16615-1-AP

IHC staining of human ovary using 16615-1-AP

IF Staining of HepG2 using 16615-1-AP

CDKN2AIP Polyclonal antibody

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CDKN2AIP is critical for spermiogenesis and germ cell development

CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1

Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.

Taurine Alleviates Pancreatic β-Cell Senescence by Inhibition of p53 Pathway

Reduced myocardial CARF and the underlying implications in left ventricular noncompaction cardiomyopathy