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Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of pig colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of pig colon using 60351-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colorectal cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colorectal cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human large intestine tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human large intestine using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human small intestine using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human appendicitis using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Cadherin-17 Monoclonal antibody

Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60351-1-Ig
Clone No.3B10A9
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

CDH17, 3B10A9, Cadherin 17, CDH16, HPT 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman colon tissue, pig colon tissue
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human colon tissue, human stomach cancer tissue, human colorectal cancer tissue, human large intestine tissue, human small intestine tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60351-1-Ig targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19488

Product name: Recombinant human CDH17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 89-406 aa of BC113464

Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA

相同性解析による交差性が予測される生物種
Full Name cadherin 17, LI cadherin (liver-intestine)
Calculated molecular weight 832 aa, 92 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC113464
Gene Symbol Cadherin-17
Gene ID (NCBI) 1015
RRIDAB_2881460
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ12864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
IHC protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
WB protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biochim Biophys Acta Gen Subj

Gastric cancer patient-derived organoids model for the therapeutic drug screening

Authors - Jiao Xu
View Article
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