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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC5L Recombinant antibody (85788-5-RR)

    WB analysis using 85788-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC5L Recombinant antibody (85788-5-RR)

    IF Staining of HepG2 using 85788-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85788-5-RR

    Affinity and Kinetic Characterization of 85788-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

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CDC5L Recombinant antibody

CDC5L Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85788-5-RR
Clone No.250089G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U2OS cells, HCT 116 cells, NIH/3T3 cells, Neuro-2a cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85788-5-RR targets CDC5L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDC5L fusion protein Ag3578 相同性解析による交差性が予測される生物種
Full Name CDC5 cell division cycle 5-like (S. pombe)
Calculated molecular weight 802 aa, 92 kDa
Observed molecular weight100 kDa
GenBank accession numberBC001568
Gene Symbol CDC5L
Gene ID (NCBI) 988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.

Protocols

Product Specific Protocols
WB protocol for CDC5L antibody 85788-5-RRDownload protocol
IF protocol for CDC5L antibody 85788-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols