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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC5L Recombinant antibody (85788-5-RR)

    WB analysis using 85788-5-RR (same clone as 85788-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85788-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC5L Recombinant antibody (85788-5-RR)

    IF Staining of HepG2 using 85788-5-RR (same clone as 85788-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85788-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85788-5-RR

    Affinity and Kinetic Characterization of 85788-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

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CDC5L Recombinant antibody, PBS Only

CDC5L Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85788-5-PBS
Clone No.250089G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85788-5-PBS targets CDC5L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDC5L fusion protein Ag3578 相同性解析による交差性が予測される生物種
Full Name CDC5 cell division cycle 5-like (S. pombe)
Calculated molecular weight 802 aa, 92 kDa
Observed molecular weight100 kDa
GenBank accession numberBC001568
Gene Symbol CDC5L
Gene ID (NCBI) 988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99459
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.