Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse thymocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CPY5-FcA65656 targets CD90.2 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A 相同性解析による交差性が予測される生物種 |
| Full Name | thymus cell antigen 1, theta |
| GenBank accession number | BC054436 |
| Gene Symbol | CD90 |
| Gene ID (NCBI) | 21838 |
| Conjugate | PerCP-Cyanine5.5 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 482 nm / 690nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01831 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP-Cyanine5.5 CD90.2 antibody CPY5-FcA65656 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |