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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human monocyte-derived mature dendritic cells using CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse I (CL750-65581)

    FC experiment of human monocyte-derived mature dendritic cells using CL750-65581

    1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL750-65581, Clone: HB15e) (red) or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

CD83 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL750-65581
Clone No.HB15e

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Synonyms

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman monocyte-derived mature dendritic cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65581 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human CD83-transfected Cos cells 相同性解析による交差性が予測される生物種
Full Name CD83 molecule
Calculated molecular weight 205 aa, 23 kDa
GenBank accession numberBC030830
Gene Symbol CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD83 antibody CL750-65581Download protocol
Standard Protocols
Click here to view our Standard Protocols