Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-SF98202 targets CD81 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Type | Rabbit / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
CatNo: Eg1036 Product name: recombinant human CD81 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: N-6*His Domain: 113-201 aa of NM_004356.4 Sequence: FVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGK 相同性解析による交差性が予測される生物種 |
| Full Name | CD81 molecule |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | NM_004356.4 |
| Gene Symbol | CD81 |
| Gene ID (NCBI) | 975 |
| ENSEMBL Gene ID | ENSG00000110651 |
| RRID | AB_3748520 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| 激发激光 | Yellow-Green Laser (561 nm) |
| 来源 | Anti-Human CD81 scFv from clone 241722G4, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus. |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 CD81 antibody CL594-SF98202 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |