Validation Data Gallery
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Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65654 targets CD71 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A 相同性解析による交差性が予測される生物種 |
| Full Name | transferrin receptor |
| GenBank accession number | BC054522 |
| Gene Symbol | CD71 |
| Gene ID (NCBI) | 22042 |
| RRID | AB_3748571 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q62351 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule, creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD71 antibody CL647-65654 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |