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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CoraLite®594-conjugated CD70 Recombinant monoclona (CL594-84455-2)

    IF Staining of Raji using CL594-84455-2

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite®594 CD70 antibody (CL594-84455-2, Clone: 241324B12 ) at dilution of 1:200.

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CoraLite®594-conjugated CD70 Recombinant monoclonal antibody

CD70 Recombinant Antibody for IF/ICC
Cat No. CL594-84455-2
Clone No.241324B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241324B12, CD27 L, CD27 ligand, CD27L, CD27-L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-84455-2 targets CD70 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD70 molecule
Calculated molecular weight 21 kDa
GenBank accession numberBC000725
Gene Symbol CD70
Gene ID (NCBI) 970
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32970
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis.

Protocols

Product Specific Protocols
IF protocol for CL594 CD70 antibody CL594-84455-2Download protocol
Standard Protocols
Click here to view our Standard Protocols