Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
SF98054-1-RR targets CD5 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Type | Rabbit / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
CatNo: Eg1119 Product name: Recombinant Human CD5 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 25-372 aa of NM_014207.4 Sequence: RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNP 相同性解析による交差性が予測される生物種 |
| Full Name | CD5 molecule |
| Calculated molecular weight | 55 kDa |
| GenBank accession number | NM_014207.4 |
| Gene Symbol | CD5 |
| Gene ID (NCBI) | 921 |
| ENSEMBL Gene ID | ENSG00000110448 |
| RRID | AB_3750145 |
| Conjugate | Unconjugated |
| 来源 | Anti-Human CD5 scFv from clone 240428A12, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus. |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD5 antibody SF98054-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |