Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-98766-2 targets CD49b in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10475 Product name: Recombinant Mouse ITA2 protein (Twin Strep Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Sequence: QSIADVAIEALFTPDKITLLNKDAKITLKLCFRAEFRPAGQNNQVAILFNMTLDADGHSSRVTSRGVFRENSERFLQKNMVVNEVQKCSEHHISIQKPSDVVNPLDLRVDISLENPGTSPALEAYSETVKVFSIPFYKECGSDGICISDLILDVQQLPAIQTQSFIVSNQNKRLTFSVILKNRGESAYNTVVLAEFSENLFFASFSMPVDGTEVTCEVGSSQKSVTCDVGYPALKSEQQVTFTINFDFNLQNLQNQAAINFQAFSESQETNKADNSVSLTIPLLYDAELHLTRSTNINFYEISSDENAPSVIKSVEDIGPKFIFSLKVTAGSAPVSMALVTIHIPQYTKEKNPLLYLTGIQTDQAGDISCTAEINPLKLPHTAPSVSFKNENFRHTKELDCRTTSCSNITCWLKDLHMKAEYFINVTTRVWNRTFAASTFQTVQLTAAAEIDTHNPQLFVIEENAVTIPLMIMKPTEKAEVPT 相同性解析による交差性が予測される生物種 |
| Full Name | integrin alpha 2 |
| Gene Symbol | CD49b |
| Gene ID (NCBI) | 16398 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD49b antibody CL488-98766-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |