Validation Data Gallery
Filter:
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
SF65651-1-AR targets CD3e in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Type | Armenian Hamster / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
N/A 相同性解析による交差性が予測される生物種 |
| Full Name | CD3 antigen, epsilon polypeptide |
| GenBank accession number | BC098236 |
| Gene Symbol | CD3ε |
| Gene ID (NCBI) | 12501 |
| Conjugate | Unconjugated |
| 来源 | Anti-Mouse CD3e scFv from clone 145-2C11, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus. |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP, 5% Trehalose, 0.01% Proclin300{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD3e antibody SF65651-1-AR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |