Validation Data Gallery
Tested Applications
| Positive FC detected in | human blood cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PY7-98145-4 targets CD34 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | CD34 molecule |
| Calculated molecular weight | 41 kDa |
| GenBank accession number | BC039146 |
| Gene Symbol | CD34 |
| Gene ID (NCBI) | 947 |
| Conjugate | PE-Cyanine7 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 450-500 nm / 778 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28906 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine7 CD34 antibody PY7-98145-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |