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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite® Plus 488-conjugated CD32 Recombinant mon (CL488-86588-2)

    IF Staining of K-562 using CL488-86588-2

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD32 antibody (CL488-86588-2, Clone: 251087D9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U-937 cells using CoraLite® Plus 488-conjugated CD32 Recombinant mon (CL488-86588-2)

    IF Staining of U-937 using CL488-86588-2

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CoraLite® Plus 488 CD32 antibody (CL488-86588-2, Clone: 251087D9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CD32 Recombinant monoclonal antibody

CD32 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-86588-2
Clone No.251087D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a

Formulation:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inK-562 cells, U-937 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86588-2 targets CD32 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2713

Product name: Recombinant Human FCGR2A/CD32a protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 34-217 aa of NM_001136219.3

Sequence: QAAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMG

相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight35 kDa
Observed molecular weight40 kDa
GenBank accession numberNM_001136219.3
Gene Symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_3747851
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
激发激光Blue laser (488 nm)
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12318-1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD32 antibody CL488-86588-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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