Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-98481-2 targets CD314/NKG2D in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3829 Product name: Recombinant Mouse CD314/NKG2D protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 90-232 aa of NM_033078.4 Sequence: FKETFQPVLCNKEVPVSSREGYCGPCPNNWICHRNNCYQFFNEEKTWNQSQASCLSQNSSLLKIYSKEEQDFLKLVKSYHWMGLVQIPANGSWQWEDGSSLSYNQLTLVEIPKGSCAVYGSSFKAYTEDCANLNTYICMKRAV 相同性解析による交差性が予測される生物種 |
| Full Name | killer cell lectin-like receptor subfamily K, member 1 |
| Calculated molecular weight | 27 kDa |
| GenBank accession number | NM_033078.4 |
| Gene Symbol | CD314 |
| Gene ID (NCBI) | 27007 |
| RRID | AB_3746799 |
| Conjugate | Biotin |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O54709-1 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In mice, CD314 is expressed on NK cells, activated CD8(+) T cells and macrophages, and subsets of TCR gamma delta (+) and NK1.1 (+) T cells (PMID: 12150888). Various subfamilies of MHC class I-related glycoproteins have been identified as the ligands for mouse CD314, including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1 (PMID: 31720075).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CD314/NKG2D antibody Biotin-98481-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |