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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    WB analysis using 86053-1-RR (same clone as 86053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of CTLL-2 cells using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    WB analysis of CTLL-2 using 86053-1-RR (same clone as 86053-1-PBS)

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02251-2

    Sandwich ELISA sample test of MP02251-2

    The mean CD3 epsilon concentration was determined to be 6.3 ng/mL in rat heart tissue extract based on a 6.7 mg/mL extract load and 13.6 ng/mL in rat spleen tissue extract based on a 2.7 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02251-2

    Sandwich ELISA standard curve of MP02251-2

    Sandwich ELISA standard curve of MP02251-2, Rat CD3 epsilon Recombinant Matched Antibody Pair - PBS only. 86053-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1450. 86053-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

Rat CD3 epsilon Recombinant monoclonal antibody, PBS Only (Detector)

CD3 epsilon Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 86053-1-PBS
Clone No.242849D3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Synonyms

CD3e, T-cell surface glycoprotein CD3 epsilon chain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86053-1-PBS targets CD3 epsilon as part of a matched antibody pair:

MP02251-2: 86053-3-PBS capture and 86053-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1450

Product name: Recombinant Rat CD3 epsilon protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-103 aa of NP_001101610.2

Sequence: IEDTVTDDGAQKQYEVSISGTSVELTCPLENEDNLKWEKNDKVLPDKNEKHLVLEDFSEVKDSGYYVCYTESSRKNTYLY

相同性解析による交差性が予測される生物種
Full Name CD3 molecule, epsilon polypeptide
Calculated molecular weight22kDa
Observed molecular weight22 kDa
GenBank accession numberNP_001101610.2
Gene Symbol Cd3e
Gene ID (NCBI) 315609
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDA6J423
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This antibody was raised against the epsilon chain of rat CD3 molecule.

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