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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 epsilon Recombinant antibody (86053-1-RR)

    WB analysis using 86053-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of CTLL-2 cells using CD3 epsilon Recombinant antibody (86053-1-RR)

    WB analysis of CTLL-2 using 86053-1-RR

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat spleen using 86053-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat spleen using 86053-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat thymus using 86053-1-RR

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IHC staining of rat thymus using 86053-1-RR

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 86053-1-RR (CD3 epsilon antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat thymus using 86053-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat thymus tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat thymus using 86053-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD3 epsilon Recombinant monoclonal antibody (86053-1-RR)

    IF Staining of rat spleen using 86053-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD3 epsilon Recombinant monoclonal antibody

CD3 epsilon Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86053-1-RR
Clone No.242849D3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD3e, T-cell surface glycoprotein CD3 epsilon chain

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat thymus tissue, CTLL-2 cells, mouse thymus tissue, rat spleen tissue
Positive IHC detected inrat spleen tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat thymus tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86053-1-RR targets CD3 epsilon in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1450

Product name: Recombinant Rat CD3 epsilon protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-103 aa of NP_001101610.2

Sequence: IEDTVTDDGAQKQYEVSISGTSVELTCPLENEDNLKWEKNDKVLPDKNEKHLVLEDFSEVKDSGYYVCYTESSRKNTYLY

相同性解析による交差性が予測される生物種
Full Name CD3 molecule, epsilon polypeptide
Calculated molecular weight22kDa
Observed molecular weight22 kDa
GenBank accession numberNP_001101610.2
Gene Symbol Cd3e
Gene ID (NCBI) 315609
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDA6J423
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This antibody was raised against the epsilon chain of rat CD3 molecule.

Protocols

Product Specific Protocols
IF protocol for CD3 epsilon antibody 86053-1-RRDownload protocol
IHC protocol for CD3 epsilon antibody 86053-1-RRDownload protocol
WB protocol for CD3 epsilon antibody 86053-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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