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Tested Applications
| Positive FC detected in | rabbit splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PY7-98349 targets CD3 in FC applications and shows reactivity with rabbit samples.
| Tested Reactivity | rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3525 Product name: recombinant rabbit CD3E+CD3G共表达 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: / 相同性解析による交差性が予測される生物種 |
| Full Name | T-cell surface glycoprotein CD3 epsilon chain |
| Gene Symbol | CD3E |
| Gene ID (NCBI) | 100008616 |
| RRID | AB_3750006 |
| Conjugate | PE-Cyanine7 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 778 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine7 CD3 antibody PY7-98349 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |