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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2AP Recombinant monoclonal antibody (86727-1-RR)

    WB analysis using 86727-1-RR (same clone as 86727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86727-1-RR (Cd2ap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD2AP Recombinant monoclonal antibody (86727-1-RR)

    IHC staining of human kidney using 86727-1-RR (same clone as 86727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD2AP Recombinant monoclonal antibody (86727-1-RR)

    IHC staining of human kidney using 86727-1-RR (same clone as 86727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD2AP Recombinant monoclonal antibody (86727-1-RR)

    IHC staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD2AP Recombinant monoclonal antibody (86727-1-RR)

    IHC staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86727-1-RR

    Affinity and Kinetic Characterization of 86727-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86727-1-RR against Mouse CD2AP were performed. The affinity constant is below 1 pM.

Mouse CD2AP Recombinant monoclonal antibody, PBS Only

CD2AP Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86727-1-PBS
Clone No.251428G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CD2 associated protein, CD2-associated protein, Mesenchyme-to-epithelium transition protein with SH3 domains 1, METS 1, Mets1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86727-1-PBS targets CD2AP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0622

Product name: Recombinant mouse Cd2ap protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-637 aa of BC157901

Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAAFLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS

相同性解析による交差性が予測される生物種
Full Name CD2-associated protein
Calculated molecular weight 637 aa, 70 kDa
Observed molecular weight74 kDa
GenBank accession numberBC157901
Gene Symbol Cd2ap
Gene ID (NCBI) 12488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9JLQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

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