Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, Daudi cells |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86727-1-RR targets CD2AP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0622 Product name: Recombinant mouse Cd2ap protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-637 aa of BC157901 Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAAFLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS 相同性解析による交差性が予測される生物種 |
| Full Name | CD2-associated protein |
| Calculated molecular weight | 637 aa, 70 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC157901 |
| Gene Symbol | Cd2ap |
| Gene ID (NCBI) | 12488 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9JLQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD2AP antibody 86727-1-RR | Download protocol |
| WB protocol for CD2AP antibody 86727-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |