Validation Data Gallery
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Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65242 targets CD1d in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse CD1d cDNA-transfected RMA-S mouse T lymphoma 相同性解析による交差性が予測される生物種 |
| Full Name | CD1d1 antigen |
| Gene Symbol | CD1d |
| Gene ID (NCBI) | 12479 |
| RRID | AB_2935024 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P11609 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD1d antibody CL647-65242 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |