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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD147 Polyclonal antibody (30880-1-AP)

    WB analysis using 30880-1-AP (same clone as 30880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30880-1-AP (CD147 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD147 Polyclonal antibody (30880-1-AP)

    WB analysis of Jurkat using 30880-1-AP (same clone as 30880-1-PBS)

    WB result of CD147 antibody (30880-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and CD147 knockout Jurkat cells. This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD147 Polyclonal antibody (30880-1-AP)

    IHC staining of human placenta using 30880-1-AP (same clone as 30880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD147 Polyclonal antibody (30880-1-AP)

    IHC staining of human placenta using 30880-1-AP (same clone as 30880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Polyclonal antibody (30880-1-AP)

    IF Staining of HeLa using 30880-1-AP (same clone as 30880-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (30880-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30880-1-PBS targets CD147 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD147 fusion protein Eg0436 相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight35-55 kDa
GenBank accession numberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35613
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).