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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD147 Polyclonal antibody (30880-1-AP)

    WB analysis of Jurkat using 30880-1-AP

    WB result of CD147 antibody (30880-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and CD147 knockout Jurkat cells.

  • Western Blot (WB) analysis of various lysates using CD147 Polyclonal antibody (30880-1-AP)

    WB analysis using 30880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30880-1-AP (CD147 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD147 Polyclonal antibody (30880-1-AP)

    IHC staining of human placenta using 30880-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD147 Polyclonal antibody (30880-1-AP)

    IHC staining of human placenta using 30880-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Polyclonal antibody (30880-1-AP)

    IF Staining of HeLa using 30880-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (30880-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO Validated

CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30880-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30880-1-AP targets CD147 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg0436

Product name: Recombinant Human CD147 protein (His Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 138-323 aa of BC009040

Sequence: EPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLA

相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight35-55 kDa
GenBank accession numberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
RRIDAB_3669773
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

Protocols

Product Specific Protocols
IF protocol for CD147 antibody 30880-1-APDownload protocol
IHC protocol for CD147 antibody 30880-1-APDownload protocol
WB protocol for CD147 antibody 30880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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