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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD146/MCAM Polyclonal antibody (17564-1-AP)

    WB analysis using 17564-1-AP (same clone as 17564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CD146/MCAM Polyclonal antibody (17564-1-AP)

    WB analysis of HepG2 using 17564-1-AP (same clone as 17564-1-PBS)

    WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD146/MCAM Polyclonal antibody (17564-1-AP)

    WB analysis using 17564-1-AP (same clone as 17564-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using CD146/MCAM Polyclonal antibody (17564-1-AP)

    WB analysis of A375 using 17564-1-AP (same clone as 17564-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CD146/MCAM Polyclonal antibody (17564-1-AP)

    WB analysis of mouse lung using 17564-1-AP (same clone as 17564-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IP experiment of HeLa using 17564-1-AP (same clone as 17564-1-PBS)

    IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IP experiment of HepG2 using 17564-1-AP (same clone as 17564-1-PBS)

    IP result of anti-CD146/MCAM (IP:17564-1-AP, 3ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IHC staining of human ovary cancer using 17564-1-AP (same clone as 17564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IHC staining of human ovary cancer using 17564-1-AP (same clone as 17564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IF Staining of mouse spleen using 17564-1-AP (same clone as 17564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IF Staining of mouse spleen using 17564-1-AP (same clone as 17564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD146/MCAM Polyclonal antibody (17564-1-AP)

    IF Staining of HUVEC using 17564-1-AP (same clone as 17564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17564-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CD146/MCAM Polyclonal antibody, PBS Only

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 17564-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17564-1-PBS targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11762

Product name: Recombinant human CD146, MCAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-350 aa of BC056418

Sequence: GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP

相同性解析による交差性が予測される生物種
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight113-120 kDa
GenBank accession numberBC056418
Gene Symbol CD146
Gene ID (NCBI) 4162
RRIDAB_2143373
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43121
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

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