Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 17564-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17564-1-AP
WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.
WB analysis using 17564-1-AP
HUVEC cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 17564-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 17564-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17564-1-AP
IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.
IP experiment of HepG2 using 17564-1-AP
IP result of anti-CD146/MCAM (IP:17564-1-AP, 3ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600ug.
IHC staining of human ovary cancer using 17564-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 17564-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HUVEC using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17564-1-AP

Publications(47)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment

Synonyms

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (47)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells
Positive IP detected in	HeLa cells, HepG2 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse spleen tissue
Positive IF/ICC detected in	HUVEC cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20000-1:80000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 19 publications below
IHC	See 12 publications below
IF	See 21 publications below
IP	See 1 publications below

Product Information

17564-1-AP targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11762 Product name: Recombinant human CD146, MCAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-350 aa of BC056418 Sequence: GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP 相同性解析による交差性が予測される生物種
Full Name	melanoma cell adhesion molecule
Calculated molecular weight	646 aa, 72 kDa
Observed molecular weight	113-120 kDa
GenBank accession number	BC056418
Gene Symbol	CD146
Gene ID (NCBI)	4162
RRID	AB_2143373
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43121
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
IF protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
IHC protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
IP protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
WB protocol for CD146/MCAM antibody 17564-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Adv Mater Spatiotemporally Programming Microenvironment to Recapitulate Endochondral Ossification via Greenhouse-Inspired Bionic Niche Authors - Xuzheng Liu View Article
human	IHC	Nat Commun CD142-positive synovial fibroblasts drive meniscus destruction in rheumatoid arthritis Authors - Hao Sun View Article
human, mouse, rat	WB,IHC,IF	Theranostics CD146(+) pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axis. Authors - Wenwen Liu View Article
mouse	IHC	Biomaterials Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate. Authors - W Benton Swanson View Article
human	IHC	Cell Death Dis Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma. Authors - Hsin Yao Chiu View Article
human	WB,IF	J Immunother Cancer CD146+ CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets Authors - Jia Chen View Article

Validation Data Gallery

WB analysis using 17564-1-AP

WB analysis of HepG2 using 17564-1-AP

WB analysis using 17564-1-AP

WB analysis of A375 using 17564-1-AP

WB analysis of mouse lung using 17564-1-AP

IP experiment of HeLa using 17564-1-AP

IP experiment of HepG2 using 17564-1-AP

IHC staining of human ovary cancer using 17564-1-AP

IHC staining of human ovary cancer using 17564-1-AP

IF Staining of mouse spleen using 17564-1-AP

IF Staining of mouse spleen using 17564-1-AP

IF Staining of HUVEC using 17564-1-AP

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spatiotemporally Programming Microenvironment to Recapitulate Endochondral Ossification via Greenhouse-Inspired Bionic Niche

CD142-positive synovial fibroblasts drive meniscus destruction in rheumatoid arthritis

CD146(+) pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axis.

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma.

CD146+ CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets

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