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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD11a (M17/4)

CD11a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

M17/4

Cat no : CL647-65177

Synonyms

Cd11a, integrin alpha L, Itgal, LFA 1, Ly 15, Ly 21

Validation Data Gallery

Applications:
  • FC experiment of C57BL/6 mouse thymocytes using CL647-65177

    FC experiment of C57BL/6 mouse thymocytes using CL647-65177

    1X10^6 C57BL/6 mouse thymocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD11a (CL647-65177, Clone:M17/4), or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were not fixed.

  • FC experiment of mouse thymocytes using CL647-65177

    FC experiment of mouse thymocytes using CL647-65177

    1X10^6 mouse thymocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD11a (CL647-65177, Clone: M17/4) (red), or 0.5 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive FC detected inmouse thymocytes
For other applications, we recommend the unconjugated version of this antibody, 65177-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen C57BL/6 mouse splenic secondary cytotoxic T cells 相同性解析による交差性が予測される生物種
Full Name integrin alpha L
GenBank accession numberBC145802
Gene symbol Itgal
Gene ID (NCBI) 16408
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD11a antibody CL647-65177Download protocol
Standard Protocols
Click here to view our Standard Protocols