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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC76 Polyclonal antibody (31484-1-AP)

    WB analysis using 31484-1-AP (same clone as 31484-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of human placenta using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of mouse testis using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC76 Polyclonal antibody (31484-1-AP)

    IF Staining of HeLa using 31484-1-AP (same clone as 31484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

CCDC76 Polyclonal antibody, PBS Only

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31484-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

tRNA [Gm4] methyltransferase, TRMT13

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31484-1-PBS targets CCDC76 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34110

Product name: Recombinant human CCDC76 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-481 aa of BC075811

Sequence: GLNSTLKDHIMSHPALHDALNDPKNGDSATKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKVTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETTA

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 76
Observed molecular weight54 kDa
GenBank accession numberBC075811
Gene Symbol CCDC76
Gene ID (NCBI) 54482
RRIDAB_3670001
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NUP7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).

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